Admin Table of Contents
- 1 Which objective lens should be used first to examine a specimen?
- 2 Which objective would you use first when focusing microscope?
- 3 Which objective lens is closest to the specimen when the specimen is in focus?
- 4 Why should you always start with the lowest objective lens?
- 5 Which focusing knob is used for each of the objectives?
- 6 What objective should be in place when the microscope is stored?
- 7 How should I focus a specimen?
- 8 Which microscope objective should I start with?
Which objective lens should be used first to examine a specimen?
Why do you need to start with 4x in magnification on a microscope? The 4x objective lens has the lowest power and, therefore the highest field of view. As a result, it is easier to locate the specimen on the slide than if you start with a higher power objective.
Which objective would you use first when focusing microscope?
Start by rotating the objective lens to lowest power. Place a slide on the stage, label side up, with the coverslip centered. On LOW POWER ONLY, use the coarse focus knob to get the object into focus.
What focusing knob should be used to start focusing the specimen?
Coarse Adjustment Knob- The coarse adjustment knob located on the arm of the microscope moves the stage up and down to bring the specimen into focus.
What do you use to bring a specimen into focus when you are just starting to look at a specimen?
to focus on a specimen at low power, lower the stage as far from the objective lens as possible and then, while looking through the microscope, bring the stage back up using the coarse adjustment knob until the specimen appears. always bring the specimen into sharp focus on low power first!
Which objective lens is closest to the specimen when the specimen is in focus?
Chapter 7
| Question | Answer |
|---|---|
| eye lens piece, lens nearest to the eye | ocular lens |
| light reflects off specimen into lens | reflected illumination |
| light passes through specimen | transmitted illumination |
| when one lens is in focus, all others lenses will be as well | profocal |
Why should you always start with the lowest objective lens?
When using a light microscope it’s important to start with the low power objective lens as the field of view will be wider, increasing the number of cells you are able to see. This makes it easier to find what you’re looking for.
Why do we need to start from the lowest objective when focusing under the microscope?
Which objective should be in place when the microscope is put away?
4X objective
Always place the 4X objective over the stage and be sure the stage is at its lowest position before putting the microscope away.
Which focusing knob is used for each of the objectives?
Focusing knobs: located on both sides of the microscope. The larger, inner coarse adjustment knob moves the stage up and down much faster and farther than the smaller, outer fine adjustment knob. The coarse adjustment knob is used ONLY with the low power (4X, 10X) objectives.
What objective should be in place when the microscope is stored?
Always place the 4X objective over the stage and be sure the stage is at its lowest position before putting the microscope away.
Which objective should you use when starting your observation of euglena?
Justify your choice of an objective to use when starting your observation of the Euglena wet mount; if the Euglena are swimming to the left, which way should you move your slide to keep the organism in view? Start with the lowest power objective so the field of view is the largest (scanning).
What are objective lenses attached to?
The revolving nosepiece is the inclined, circular metal plate to which the objective lenses, usually four, are attached. The objective lenses usually provide 4x, 10x, 40x and 100x magnification. The final magnification is the product of the magnification of the ocular and objective lenses.
How should I focus a specimen?
When focusing a specimen, you should always start with the SCANNING OBJECTIVE. The scanning power objective lens will magnify the specimen by a factor of 4, making it possible for you to view the specimen clearly.
Which microscope objective should I start with?
Which Microscope Objective Should I Start With? Start Low! Since the 4x objective lens has the least magnification, but a larger field of view, it allows for more of the specimen to be seen, as well as locating the part of the sample you wish to view.
Why should you start with the lowest magnification objective when getting a specimen?
Why Should You Start With the Lowest Magnification Objective When Getting a Specimen in Focus? Microscopes can be tricky to use because of the strength of their lens magnifications, typically 4x, 10x and 40x.
Why are microscopes so difficult to use?
Microscopes can be tricky to use because of the strength of their lens magnifications, typically 4x, 10x and 40x. The difficulty lies in trying to align the specimen in a precise position so the microscope lens can capture it and allow the user to see the specimen close up.