Admin Table of Contents
- 1 What medium is essential for development of discrete isolated colonies?
- 2 What is the term for a culture made from one isolated colony?
- 3 What type of isolation technique is most effective for most lab applications?
- 4 How is an isolated colony formed?
- 5 What is the importance of isolated colonies?
- 6 Why is it important to isolate and identify bacteria?
What medium is essential for development of discrete isolated colonies?
Cards
| Term The five I’s of studying microorganisms include all of the following except | Definition infection |
|---|---|
| Term Which of the following is essential for development of discrete, isolated colonies? | Definition solid medium |
| Term A pure culture contains only | Definition one species of microorganisms |
What type of media would be the best choice for isolating a bacterial colony?
EMB agar is a medium used in the identification and isolation of pathogenic bacteria. It contains digested meat proteins as a source of organic nutrients.
What is the term for a culture made from one isolated colony?
pure culture, in microbiology, a laboratory culture containing a single species of organism. Both methods separate the individual cells so that, when they multiply, each will form a discrete colony, which may then be used to inoculate more medium, with the assurance that only one type of organism will be present.
Which technique’s is are used to help identify and classify bacteria?
DNA-based approaches used in the identification and classification of species of bacteria include DNA-DNA hybridization, DNA fingerprinting, and DNA sequencing. DNA-DNA hybridization, initially developed in the 1980s, is used to determine the similarity of DNA sequences from different organisms.
What type of isolation technique is most effective for most lab applications?
The streak plate technique is the most popular method for isolating specific bacteria from a sample containing a mixture of microorganisms. The technique essentially dilutes the number of organisms and reduces their density. It allows microbiologists to distinguish and isolate individual bacterial colonies.
What is incorrect about chocolate agar?
Which is incorrect about chocolate agar? It has chocolate extract in it.
How is an isolated colony formed?
When you inoculate your media, you introduce bacterial cells into a nutrient-rich environment. When these lone bacterial cells divide and give rise to thousands and thousands of new bacterial cells, an isolated colony is formed. Ideally, an isolated colony of bacteria is the progeny of a single bacterial cell.
What is the purpose of selective media?
Selective and differential media are used to isolate or identify particular organisms. Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms.
What is the importance of isolated colonies?
A pure, isolated colony is what scientists want to start with before beginning an experiment; it allows for consistency and assurance that your sample isn’t contaminated with any other strains of bacteria.
Why is it important to get isolated colonies?
Since all of the cells in a colony derive from a single original cell through repeated binary fission; all of the cells in that colony should be genetically identical. Therefore an ISOLATED colony represents a pure source of an organism from which a pure culture can be started.
Why is it important to isolate and identify bacteria?
In microbial ecology, the identification of microorganisms helps us characterize biodiversity. Because the clinical samples will most likely contain many microorganisms, both normal flora and pathogens, it is important to isolate the pathogen in a pure culture using various types of selective and differential media.
Why is it important to characterize and identify unknown environmental bacteria?
WHY IS THE IDENTIFICATION OF BACTERIAL UNKNOWNS IMPORTANT? Microbiologists must identify bacterial isolates for several practical reasons: • Medical diagnostics — identifying a pathogen isolated from a patient. Food industry — identifying a microbial contaminant responsible for food spoilage.