What happens when DCPIP is reduced?

What happens when DCPIP is reduced?

When oxidized, DCPIP is blue with a maximal absorption at 600 nm; when reduced, DCPIP is colorless. DCPIP can be used to measure the rate of photosynthesis. As DCPIP is reduced and becomes colorless, the resultant increase in light transmittance can be measured using a spectrophotometer.

Why is DCPIP a good indicator?

DCPIP can not only indicate redox potential, but also pH. Under some conditions it is pink in acid pH. “This assay takes advantage of the fact that ascorbic acid is a good reducing agent. If a drop of blue DCPIP dye is added to a low pH solution (pH<4.0), it will turn pink (reaction 1).

Is Dichloroindophenol safe?

Eyes: May cause irritation. Skin: May cause irritation to skin. Ingestion: May cause gastrointestinal discomfort. Inhalation: May cause irritation to respiratory tract.

Is ascorbic acid hazardous?

Ingestion: Ingestion of large amounts may cause gastrointestinal irritation. Low hazard for usual industrial handling. Large doses may cause diarrhea and acidification of the urine which may cause stones in the urinary tract. Inhalation: May cause respiratory tract irritation.

What is DCIP used for?

2,6-Dichlorophenolindophenol (DCPIP, DCIP or DPIP) is a chemical compound used as a redox dye. When oxidized, DCPIP is blue with a maximal absorption at 600 nm; when reduced, DCPIP is colorless.

Is ammonium hydroxide hazardous?

Ammonium hydroxide causes skin irritation; contact can lead to severe irritation and burns. Ingestion can cause vomiting, nausea, gastric irritation and, in severe cases, perforation, central nervous system depression, shock, convulsions and pulmonary edema.

Is Indophenol safe?

Not a dangerous substance or mixture according to the Globally Harmonized System (GHS). Potential Health Effects: Skin: May be harmful if absorbed through skin.

What does ascorbic acid react with?

It typically reacts with oxidants of the reactive oxygen species, such as the hydroxyl radical. On exposure to oxygen, ascorbic acid will undergo further oxidative decomposition to various products including diketogulonic acid, xylonic acid, threonic acid and oxalic acid.

What replaces DPIP in photosynthesis?

Since DPIP replaces NADPH in the light reactions, it will turn from blue to colorless when reduced during photosynthesis. This will allow you to monitor the rate of photosynthesis.

What happens to the absorbance of DCPIP when it is reduced?

DCPIP is a blue colour when its oxidized and when it is reduced it turns into a colourless solution. DCPIP replaces NADPH the final electron acceptor in the light dependent reaction. So basically, as the reaction continues, the more DCPIP is reduced and the absorbance decreases.

Does the DCPIP decolourise when exposed to light?

This shows that the DCPIP does not decolourise when exposed to light. Tube 3 (leaf extract + DCPIP in the dark) no colour change. It can therefore be inferred that the loss of colour in tube 1 is due to the effect of light on the extract. Tube 4 (leaf extract + distilled water) no colour change.

How can I tell the difference between NADP and DCPIP?

You should observe that the solution of the DCPIP in test tube A has completely decolourised whilst test tube B still has most, if not all, of its original blue colour. DCPIP takes the place of NADP in this set-up.

How do you know if DCPIP has accepted electrons?

The reaction can only occur if the thylakoid membranes are illuminated as the light-dependent stage stops in the dark. DCPIP is blue when oxidised (at pH 7.0) and colourless when reduced, so it is possible to monitor the loss of blue colour as an indication that DCPIP has accepted electrons.