Admin What does calibration refer to in HPLC?
Calibration refers to the process of determining the relation between the output (or response or signal) of a measuring instrument and the value of the input quantity or property.
How do you measure injector accuracy?
Injector Precision PRECISION CALCULATION: %RSD for retention time and %RSD for peak area are calculated by dividing the standard deviation of the peak area or the standard deviation of the retention time by the average of the peak area or the average of the retention time multiplied by 100.
How do I calibrate autosampler?
Place the calibration fixture in position over the sample and reference positions. Select AUTOSAMPLER from the Control Menu Touch Screen. Then select CELL CAL from the Autosampler Control Touch Screen. The calibration will proceed automatically.
Why caffeine is used for calibration of HPLC?
Caffeine is convenient because it is not volatile, readily available, safe, has strong UV adsorption if you are using UV detector, and does not retain too long on RP columns (faster calibration).
What is wavelength accuracy in HPLC?
Depending on the regulations or. guidelines applied, most authorities require accuracy to be within 2 to 3 nm of a certified. standard within the range used. In practice, we generally achieve accuracy of equal to or. better than 0.5 nm across a range of UV / VIS wavelengths.
What is injector linearity?
The ability of an injector to vary the injected volume in direct proportion to the requested volume is its linearity. (Note: This experiment was designed so that the amount of injected material was within the linear range of the Waters 2487 detector.)
How do you calculate injection volume HPLC?
For example, if you are currently injecting 20 µL on a 150 x 4.6 mm column and then switch to a 150 x 3.0 mm column, you could estimate the adjusted volume by multiplying 20 x (1.52)/(2.32). Your new volume should be about 8.5 µL.
What is the tailing factor in HPLC?
The Tailing Factor is defined by the USP as the distance from the front edge of the peak to the back edge, divided by the distance from the front edge to the centerline, with all distances measured at 5% of the maximum peak height.
What is tailing factor in HPLC?
Tailing Factor (Tf) is the USP coefficient of the peak symmetry.