What does a tailing do?

What does a tailing do?

Tailing is an enzymatic method for adding a non-templated nucleotide to the 3′ end of a blunt, double-stranded DNA molecule. Tailing is typically done to prepare a T-vector for use in TA cloning or to A-tail a PCR product produced by a high-fidelity polymerase (not Taq) for use in TA cloning.

What should be done with tailings?

Tailings as a resource Another productive means of utilising tailings is to create more sustainable by-products. This includes everything from commercial shotcrete and concrete products for self-sustaining uses such as mine roads, brick and tile manufacture, insulation, or even foamed products.

How tailing are formed?

Tailings consist of ground rock and process effluents that are generated in a mine processing plant. Mechanical and chemical processes are used to extract the desired product from the run of the mine ore and produce a waste stream known as tailings. The challenges associated with tailings storage are ever increasing.

Which enzyme is responsible for tailing?

The best-described role of 3′ tailing is the bulk polyadenylation of messenger RNAs in the cell nucleus that is catalyzed by canonical poly(A) polymerases (PAPs). However, many other enzymes that add adenosines, uridines, or even more complex combinations of nucleotides have recently been described.

How can we reduce tailings?

Here are three of the more common alternatives, which can also reduce or avoid the need for water-covered TSFs: Paste or thickened tailings on surface – One way to help reduce the amount of water going to the TSF is through thickening the tailings, so that the product moves through the pipe in a plug-flow rather than a …

What are the concerns of tailings?

There are several environmental concerns that are associated with the existence of tailings ponds. The main problem with the ponds is that they include toxic and harmful chemicals such as ammonia, mercury, and naphthenic acids. The water containing these chemicals is toxic to animals, particularly aquatic organisms.

What are tailing ponds used for?

Tailings ponds are engineered dam and dyke facilities used for storage of tailings materials. Tailing ponds are also used to enable water to separate from the tailings. Water from the tailings ponds is recycled back into the extraction process, reducing the use of fresh water from the Athabasca River and other sources.

How do tailing ponds work?

What is coal tailing?

Coal refuse (also described as coal waste, coal tailings, waste material, culm, boney, or gob) is the material left over from coal mining, usually as tailings piles or spoil tips.

What is deposition mine tailing?

6.5 Tailings Impoundments. Mine tailings are the finely ground residue from ore extraction. The method of deposition affects the distribution of tailings particles within the impoundment.

How is poly A tail added?

The poly-A tail is a long chain of adenine nucleotides that is added to a messenger RNA (mRNA) molecule during RNA processing to increase the stability of the molecule. Immediately after a gene in a eukaryotic cell is transcribed, the new RNA molecule undergoes several modifications known as RNA processing.