How do you clean the inside of a pipette?

How do you clean the inside of a pipette?

Immerse the contaminated parts in a detergent solution such as Deconex® 12 Basic. Rinse thoroughly with distilled water and allow to dry. Open the pipette and place the contaminated parts in a strong detergent or cleaning solution. Rinse several times with distilled water and allow to dry.

How do you clean and reuse a pipette?

They used to put them in soap for a couple of hours, then wash them abundantly with tap and distilled water, boil them for 2 minutes and dry them at 37C. For basic rules of safety and possible cross contamination pipette tips should not be re-used.

How do you care for a pipette?

Storage

1. Store pipettes vertically to avoid contamination.
2. Store pipettes dialed to highest volume setting.
3. Store away from heat and moisture.
4. Pipette tips should be removed prior to storage.
5. Keep pipettes clean and free of contaminants.

What happens if you use a dirty pipette?

Using a dirty pipet causes too little or contaminated solution to be delivered. Leaving little droplets behind on the walls (except for the small amount in the tip) causes too little solution to be delivered. Forcing the pipet into the bulb usually causes liquid to be sucked into the bulb.

What is used to rinse a pipette?

When you are finished with the pipet in your experiment, rinse with distilled water.

How do you make a pipette sterile?

The Best Recipe for Sterilizing Pipette Tips

1. Open the lid of the tip tray a tad—propping it up with the lid’s locking tab helps; a little tape can hold it in place—don’t wrap the box in foil!
2. Run your dry cycle as normal.
3. Let the damp tips dry in the warm autoclave (or transfer to a drying cabinet/incubator)

Can pipette tips be washed and reused again?

Composed of polypropylene, a highly recyclable plastic, pipette tips can now be reused multiple times with the integration of Grenova tip reuse technology in laboratory workflows. On average, a single tip can be washed, sterilized, and reused between 10-25 times.

How do you clean a volumetric pipette?

Cleaning Pipets and Volumetric Flasks

1. Clean pipets and volumetric flasks using warm soapy water.
2. Rinse with tap water followed by three to four rinses with deionized water. This water rinse should form a smooth sheet when poured through clean glassware.
3. Dry using acetone to remove water.

How do you sterilize plastic pipette tips?

How can pipette contamination be prevented?

Prevention:

1. Keep the pipette vertical when pipetting in order to prevent liquid from running into the pipette body.
2. Release the push button slowly.
3. To avoid aerosol contamination, use filter tips or use a positive displacement pipette and tips.

How do I clean the pipette?

Please make sure that the pipette is clean and that any residuals of the cleaning agent have been removed. Autoclave the pipette at 121°C and 1 bar overpressure for 20 minutes. Afterwards, cool the pipette to ambient temperature and let it dry.

How do I disinfect my Eppendorf pipettes?

UV-disinfection of pipettes: The Eppendorf Pipettes may be temporarily subjected to UV light under a sterile bench (see the operating manual for pipettes of other manufacturers). We recommend the use of a Hg-lamp, 254 nm, 30 W.

How to lubricate the piston of a pipette?

Lightly lubricate the piston with silicone grease (usually provided with your pipette) and reassemble the pipette. And don’t forget the O-ring! Check the calibration of your pipette before using it again. Happy pipetting! This site uses Akismet to reduce spam.

How can I Clean my pipette after DNA extraction?

Boil the lower parts of the pipette in glycine/HCI buffer (pH 2) for 10 minutes. Rinse with distilled water. Air dry as above. You can also use UV light to further reduce DNA contamination.