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At what pH Did you see the highest activity of salivary amylase Why?
At pH 7 the highest activity of salivary amylase occurred. The IKI test shows that pH 7 is optimal for activity because the most starch was digested here (a negative result) and the Benedict’s Test showed the amount of glucose that was broken down from the starch and it yielded the most glucose (+2).
What pH would result in the highest amylase activity?
Result: The highest lipase activity was found under 37 degrees C, pH 8.2, and the highest amylase activity was detected under 37 degrees C, pH 5.2, while protease activity peaked at 42 degrees C, pH 3.2 or pH 9.2.
Why is optimum pH of amylase 7?
The enzyme amylase breaks down starch into glucose. If the enzyme is working effectively, this will happen quickly. This shows that the starch was broken down more quickly at this pH. The optimum pH for amylase is therefore pH 7.
What is the optimum pH for salivary amylase?
7.0
Results: In vitro activity of human salivary alpha-amylase showed the optimum pH and temperature at 7.0 and 37 degrees C, respectively.
What is the pH of salivary amylase?
pH 6.8
The optimum pH for the action of salivary amylase is pH 6.8.
How pH affects amylase activity?
The optimum pH for the reaction of starch with amylase is pH 7. PH values lower or higher than this value will result in a slower rate of reaction. Amylase works in the range pH 3 to pH 11. PH changes affect the structure of an enzyme molecule and therefore affect its ability to bind with its substrate molecules.
What pH level is amylase?
6.7 – 7.0
Effects of pH
| Enzyme | pH Optimum |
|---|---|
| Maltase | 6.1 – 6.8 |
| Amylase (pancreas) | 6.7 – 7.0 |
| Amylase (malt) | 4.6 – 5.2 |
| Catalase | 7.0 |
Which pH does salivary amylase work best at?
∼7.0
The acinar cells produce an enzyme called ptyalin, or salivary amylase, which is involved in the digestive process initiating the hydrolysis of starch present in food. The pH for optimal activity of ptyalin is ∼7.0 and it requires the presence of Cl−.
How pH affect salivary amylase?
Effect of pH The optimum pH for the enzymatic activity of salivary amylase ranges from 6 to 7. Above and below this range, the reaction rate reduces as enzymes get denatured. Our stomach has a high level of acidity which causes the salivary amylase to denature and change its shape.
How does pH affect activity of amylase?
As can be seen above, the optimum pH for the enzyme Salivary Amylase is around 7. As the pH distances from the optimum, however, the reaction rate decreases because the shape of the enzyme’s active site begins to deform, until it becomes denatured and the substrate can no longer fit the active site.