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What does diphenylamine react with?
The deoxyribose in DNA in the presence of acid forms β-hydroxylevulinaldehyde which reacts with diphenylamine to give a blue colour with a sharp absorption maximum at 595nm. In DNA, only the deoxyribose of the purine nucleotides react, so that the value obtained represents half of the total deoxyribose present.
What is the chemical reaction involved in diphenylamine test?
DNA can be identified chemically with the Dische diphenylamine test. Acidic conditions convert deoxyribose to a molecule that binds with diphenylamine to form a blue complex. The intensity of the blue color is proportional to the concentration of DNA. The amount of blue corresponds to the amount of DNA in solution.
What is the purpose of diphenylamine test?
This is a color test for the presence of nitrates or nitrites. Use caution! This reagent contains concentrated sulfuric acid (H2SO4).
Is diphenylamine soluble in water?
Diphenylamine dissolves well in many common organic solvents, and is moderately soluble in water. It is used mainly for its antioxidant properties.
Is RNA detected by diphenylamine?
RNA has been demonstrated to react with diphenylamine when acid hydrolysis is performed for 1 hour or more at 100°C. In this way, diphenylamine can be used for the simultaneous determination of the concentrations of DNA and RNA in mixtures.
Is diphenylamine flammable?
FIRE HAZARDS * Diphenylamine may burn, but does not readily ignite. * Use dry chemical, CO2, water spray, alcohol or polymer foam extinguishers.
Is the diphenylamine test a conclusive test?
The (Diphenylamine or Parrafin) test is not conclusive as to the presence of gunpowder because fertilizers, cosmetics, cigarettes, urine, and other nitrogenous compounds with nitrites and nitrates will give a positive reaction. This (Diphenylamine or Parrafin) test has proved extremely unreliable in use.
Is diphenylamine test conclusive?
What are the properties of diphenylamine?
The other properties of diphenylamine include: Density: 1.16; Melting point: 52-54 ºC; Boiling point: 302 ºC; Flash point : 152 ºC; Water solubility: Slightly soluble. 0.03 g/100 mL. Diphenylamine is often used for producing stabilizer of explosive and fuel.
Is diphenylamine soluble in milk?
Diphenylamine has been detected in milk of animals (cow, sheep, goat, water buffalo) raised in Italy and France. Solubility in water is 25.8 mg l −1, while solubility in organic solvents is considerably higher.
What is the formula for diphenylamine redox?
Diphenylamine is also known as Diphenylamine redox, with its CAS No. 122-39-4, and it has the molecular formula of C 12 H 11 N and a molecular weight of 169.22. The other properties of diphenylamine include: Density: 1.16; Melting point: 52-54 ºC; Boiling point: 302 ºC; Flash point : 152 ºC; Water solubility: Slightly soluble. 0.03 g/100 mL.
How do you make diphenylamine from aniline?
Diphenylamine is prepared by heating under pressure aniline hydrochloride with a slight excess of aniline. The excess aniline may be extracted from the product by means of dilute hydrochloric acid and the very weakly basic diphenylamine is purified by distillation.