What can be used to determine the precise amount of radioactively labeled DNA in a chemical solution?

What can be used to determine the precise amount of radioactively labeled DNA in a chemical solution?

Autoradiography is used for detecting radioactively-labeled DNA or RNA in a gel after separation by electrophoresis. Electrophoresis gels containing RNA or DNA are transferred onto membranes by blotting to allow more convenient handling during autoradiography, or the gel can be dried onto filter paper.

Which of the following can be used to determine the precise amount of radioactively?

Geiger counters are commonly used to measure the amount of radioactivity, but there are other types of detectors that may be used.

What enzyme is used to make radioactively labeled DNA?

The nick translation reaction is used to introduce radioactive nucleotide phosphates into unlabeled DNA for the purpose of making a probe. The reaction depends on the ability of the enzyme DNA polymerase I to initiate DNA synthesis at free 3´ OH groups, which are exposed as nicks in the unlabeled DNA.

Which tool is used to determine the presence of radioactively labeled DNA apex?

In molecular biology, a hybridization probe is a fragment of DNA or RNA of variable length (usually 100–10000 bases long) which can be radioactively or fluorescently labeled.

Which of the following reactions is used to radioactively label DNA?

Nick translation is a technique for radioactively labeling double-stranded DNA, making it suitable as a hybridization probe for detecting specific genomic sequences.

Which of the following best describes the experiment by Kornberg that led to an understanding that DNA polymerase required a template for DNA synthesis?

Facilitate base pairing between single stranded molecules of DNA. Which of the following best describes the experiment by Kornberg that led to an understanding that DNA polymerase required a template for DNA synthesis? He observed that DNA synthesis in bacterial cell extracts required all four dNTPs.

How do you radioactively label DNA?

To radioactively label a DNA fragment for use as a probe, one of the incorporated nucleotides provided in the reaction is radiolabeled on the alpha phosphate position. The translated nick can be sealed by DNA ligase.

Why is it important that one of the dNTPs is radioactively Labelled?

One or more of the dNTPs are radioactively labeled to help visualize the replication products. Finally, each tube gets one of four special nucleotides called dideoxynucleotides (ddNTPs). These nucleotides are recognized and used by DNA polymerase, and can be incorporated at the 3′ end of a growing chain.

What is the strand that is copied discontinuously because it is traveling away from helicase?

The “lagging strand” is synthesized in the direction away from the replication fork and away from the DNA helicase unwinds. This lagging strand is synthesized in pieces because the DNA polymerase can only synthesize in the 5′ to 3′ direction, and so it constantly encounters the previously-synthesized new strand.

What is DNA’s significance when learning about its role in evolution?

DNA contains genes, the building blocks of all organisms. The most important function of DNA is its ability to replicate itself repeatedly. DNA must be copied when new cells are formed, when genetic material is passed from parents to offspring, and when coding for RNA (ribonucleic acid) to make proteins.

What are radioactively Labelled probes?

To radioactively label a DNA fragment for use as a probe, one of the incorporated nucleotides provided in the reaction is radiolabeled on the alpha phosphate position. In this reaction, RNA is reverse transcribed to cDNA using reverse transcriptase.