Why is the inoculating loop flamed with the Bunsen burner?

Why is the inoculating loop flamed with the Bunsen burner?

A loop or needles is sterilized by inserting it into a Bunsen burner flame until it is red hot. This will incinerate any contaminating organisms that may be present. Allow the loop to cool completely beefore picking up inoculum. This will ensure that viable cells are transferred.

When handling a burning inoculation loop or other instrument where and when should the instrument be put down?

A) It is necessary to hold the inoculating instrument in the Bunsen burner flame until it becomes red hot. Once you have sterilized your inoculating loop, it is important that you wait 10-20 seconds before using the loop to pick up a sample from your culture.

How should you heat an inoculating loop in the Bunsen burner flame to ensure sterility?

How should you heat an inoculating loop in the Bunsen burner flame to ensure sterility? After flaming the loop or the needle to sterilize it, you will need to allow it to cool for a few seconds so that the heat will not kill or damage the bacteria you are about to pick up with it out of the broth culture.

What is a flame loop used for?

Flame sterilization is a very quick simple method of killing microorganisms on an inoculating loop or needle. The loop or needle is held inside a flame for a few seconds to bring it to redness and then cooled. Once cool, the loop or needle can be used for various culture manipulations.

Why is the loop flamed before it is placed in a culture tube Why is it flamed after completing the inoculation?

Why it it flamed after completing the inoculation? The loop or the tube is flamed to sanitize tools from bacteria. The plate should always be inverted during incubation that way we do not prevent formation of colonies on the agar surface otherwise the water that condensates will spread the bacteria along the surface.

How do you hold an inoculating loop?

Hold the inoculating loop with your thumb and first two fingers. Heat the inoculating loop in the Bunsen burner until it is red hot. Heat several inches of the loop, since that much of it will contact the inside of the tubes. Allow the loop to cool for a few seconds while you hold it in your hand.

How should you hold the inoculating loop quizlet?

How do you hold an inoculation loop? Hold it just like you hold the pencil, using your dominant hand.

What is the purpose of flame sterilizing the inoculating loop needle before and after using it?

For each portion of the streak (3 total per plate), flame-sterilize the inoculating loop just prior to use. Also, flame-sterilize the loop just after the final streak is performed in order to prevent contamination of the bench surface and as a consideration to others in the lab who may later use the inoculating loops.

How do you use a bunsen burner for agar plates?

Make sure to place agar plates in the incubator upside down. This prevents water condensation from dripping onto the agar surface and spreading over the colonies. This will also prevent contamination and aid in the formation of isolated colonies. Properly adjust the flame of the Bunsen burner.

How do you sterilize a wire inoculating loop?

Sterilize your wire inoculating loop by passing it at an angle through the flame of a Bunsen burner until the entire length of the wire becomes glowing red/orange from the heat. Important: Never lay the loop down once it is sterilized or it may become re-contaminated.

Why do you put the culture tube in the opposite direction?

Because flaming in this direction limits aerosol production by allowing the tip to heat up more slowly than if it were put into the flame immediately. When making a transfer, which should you move; the culture tube in your nondominant hand or the loop in your dominate hand?

What is the purpose of the flaming of the loop?

The flaming of the loop at the points indicated is to effect the dilution of the culture so that fewer organisms are streaked in each area, resulting in the final desired separation. Remove the loop and close the Petri dish. Tape the plate closed and incubates the plate in an inverted position in an incubator for 24-48 hours.

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