Admin Table of Contents
How a solution is drawn into a pipette by using a pipette filler?
Siphon liquid into the pipette to the desired level by squeezing valve “S” on the bottom of the pipette filler. This uses the vacuum created in the bulb to draw liquid into the pipette. Be careful not to draw liquid into the pipette filler. This allows you to release liquid at the desired rate and to the desired level.
Why is a rubber safety bulb or pipet filler always used to fill a pipet?
It serves as a vacuum source for filling reagents through a pipette or pasteur pipette and also help control the flow of liquid from the dropping bottle. By using rubber bulb, the contact of the mouth to the chemicals can be avoided.
When inserting the pipette filler or bulb to the pipette one must hold the pipette at?
Regardless of the type of pipette filler, hold the pipette at the top and carefully insert it into the filler by pushing gently. 1. Hold the pipette at the top and carefully insert it into the rubber bulb filler.
What is a pipette filler used for in titration?
Titrations are a very accurate experimental technique used to find volumes of acid and alkali solutions that react together. A safety pipette filler should be used to draw up the solution until the bottom of the meniscus is on the line and the solution is then released into a conical flask. …
Why is a rubber safety bulb always used when measuring volumes with a pipet?
Why is a rubber safety bulb always used when working with a pipet? Because it creates suction without using your mouth to draw up liquid, which was a practice previously used but is now considered unsafe. TD means to deliver, meaning that the pipet or buret will deliver that certain amount.
What can you not do with a pipet?
10 Ways to Abuse a Pipette
- Forgetting your head. When using a pipette, always use a tip.
- The wrong guy for the job. Make sure your tip is the right one for the volume you are pipetting.
- Pushing it too far.
- It’s a pipette, not a plunger.
- Leaving it lying around.
- Using it as a backscratcher.
- Holding it wrong.
- Dialing it up.
How do you transfer a liquid to a pipette?
Apply suction until liquid is withdrawn to just past the desired mark. Remove the pipette bulb and place your finger atop the pipette. Allow tiny amounts of air to be let into the top of the pipette by wiggling your finger or a slight release of pressure. Drain the liquid to the desired mark.
How do I learn to pipette?
Make sure to press the tip firmly onto the pipette, because a loose tip will not draw the proper volume of liquid. Slowly depress the plunger before submerging the tip into the liquid. Submerge the tip 3 to 4 mm into the liquid. The more you submerge, the more liquid may stick to the outside of the tip.