Table of Contents
Why do we always start focusing with low power instead of high power?
Changing from low power to high power increases the magnification of a specimen. The amount an image is magnified is equal to the magnification of the ocular lens, or eyepiece, multiplied by the magnification of the objective lens. Usually, the ocular lens has a magnification of 10x.
How do you focus a microscope at high power?
Play with the magnification using the coarse adjustment knob and move your slide around until it is centered. When switching to a higher-power objective, focus using the fine adjustment knob until the edges, and specific features of interest become sharp and clear.
How do you focus a microscope on low power?
- Start by rotating the objective lens to lowest power.
- Place a slide on the stage, label side up, with the coverslip centered.
- On LOW POWER ONLY, use the coarse focus knob to get the object into focus.
- If you cannot see anything, move the slide slightly while viewing and focusing.
Why must you center and focus the object under low power?
Why must you center and focus the object in the field of view under low power before switching to high power? When you switch to high power, you are focusing on a very small part of the large picture.
How do I use the fine focus and coarse focus?
Focus using the coarse adjustment knob first, then make adjustments with the fine adjustment knob later on higher power objectives. If you want to go to a higher power objective, do so, then focus again with the fine focus knob only.
How do I focus on a slide?
Focusing on a slide takes patience. You may find yourself tempted to turn to the highest power objective and start twisting both focus knobs right away. But resist the temptation. Whether focusing on a dry or wet mount slide, always start on the lowest power objective.
Why won’t my microscope focus on my highest power objective?
If you have engaged your highest power objective but are unable to bring it into focus that is because typically the highest power objective on a microscope is a 100X oil immersion objective.